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| Schmitt, I.; Crespo, A.; Divakar, P.K.; Fankhauser, J.D.; Herman-Sackett, E.; Kalb, K.; Nelsen, M.P.; Nelson, N.A.; Rivas-Plata, E.; Shimp, A.D.; Widhelm, T.; Lumbsch, H.T.. |
| Developing powerful phylogenetic markers is a key concern in fungal phylogenetics. Here we report degenerate primers that amplify the single-copy genes Mcm7 (MS456) and Tsr1 (MS277) across a wide range of Pezizomycotina (Ascomycota). Phylogenetic analyses of 59 taxa belonging to the Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes and Sordariomycetes, indicate the utility of these loci for fungal phylogenetics at taxonomic levels ranging from genus to class. We also tested the new primers in silico using sequences of Saccharomycotina, Taphrinomycotina and Basidiomycota to predict their potential of amplifying widely across the Fungi. The analyses suggest that the new primers will need no, or only minor sequence modifications to amplify... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Ascomycota; DNA replication licensing factor; Evolution; Lichenised fungi; Mcm7; MS277; MS456; Phylogeny; Pre-rRNA processing protein; Protein-coding; Tsr1. |
| Ano: 2009 |
URL: http://www.repository.naturalis.nl/record/531799 |
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| Mardones, M.; Trampe-Jaschik, T.; Oster, S.; Elliott, M.; Urbina, H.; Schmitt, I.; Piepenbring, M.. |
| The order Phyllachorales (Pezizomycotina, Ascomycota) is a group of biotrophic, obligate plant parasitic fungi with a tropical distribution and high host specificity. Traditionally two families are recognised within this order: Phyllachoraceae and Phaeochoraceae, based mostly on morphological and host characteristics. Currently, the position of the order within the class Sordariomycetes is inconclusive, as well as the monophyly of the order, and its internal phylogenetic structure. Here we present a phylogeny of the order Phyllachorales based on sequence data of 29 species with a broad host range resulting from a wide geographical sampling. We inferred Maximum Likelihood and Bayesian phylogenies from data of five DNA regions: nrLSU rDNA, nrSSU rDNA, ITS... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Ancestral state reconstruction; Plant parasitic; Tar spot fungi; Telimenaceae. |
| Ano: 2017 |
URL: http://www.repository.naturalis.nl/record/630890 |
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